Anti-Human IFN Beta Antibody, Clone MMHB-2 (MAb)
- Specifications
- Tech Info / Data
- Citations
- Documentation
Specifications
| Formulation | Supplied lyophilized in phosphate-buffered saline (PBS) containing 5% trehalose prior to lyophilization |
| Antigen | Human interferon beta |
| Isotype | IgG1 |
| Host Species | Mouse |
| Purity | Determined by protein A or G affinity chromatography |
| Bioactivity | Measured by neutraliziation of interferon in antiviral assay; ND50 on human (HeLa/EMCV) cells is ~7-21 μg/ml in the presence of 10 ng/ml of human IFN-beta. |
| Specificity | Neutralizes human IFN-beta; does not neutralize human IFN-alpha or gamma |
| Storage | For retention of full activity store at -20°C or below and avoid repeated freeze/thaw cycles |
| Antigen Synonyms | Human Beta Interferon, Human IFNB, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B |
| Clone | MMHB-2 |
Tech Info / Data
Recommended Applications:
- Neutralization
- Direct ELISA: 0.5 - 1.0 μg/ml
- Western Blot: 1 - 2 μg/ml
Citations
Documentation
21410-1 Certificate of Analysis (CoA) SDSNeutralization of Hu-IFN-β
To measure the ability of the antibody to neutralize the bioactivity of the human interferon beta on HeLa cells, Hu-IFN-ß was added to various concentrations of the antibody. The antigen-antibody mixture was added to confluent cultures of the HeLa cells in a 96-well plate. The assay mixture in a total volume of 100 µl, containing antibody at the concentrations as indicated, Hu-IFN-ß at 10 ng/ml, was incubated at 37ºC for 20-24 hours in a humidified CO2 incubator. At the end of this incubation period, medium was aspirated from all wells and an appropriate titrated amount of the EMCV in pre-warmed culture medium was added to each test well. After another 20-24 hour incubation, the cells were fixed, stained and scored for cytopathic effect by measurement of optical densities in a microplate reader at 540 nm. The ND50 of the antibody is approximately 7-21 µg/ml.
