Human IL-28B/IFN Lambda 3, HCE

Product Features:

Only Human IFN Lambda proteins qualified against the international reference standard for Human Interferon Lambda 1 (Hu-IFN-λ1)
Packaged and sold by active units rather than mass
Authentic human cell origin

Full Product Name: Human Interleukin-28B/Interferon Lambda 3, Human Cell-Expressed (HCE)

Catalog No.	Pack Size	Price	Quantity
11730-1	≥ 1 x 10⁵ units	$270.00

Specifications
Tech Info / Data
Citations
Documentation

Specifications

Formulation	Supplied frozen in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Molecular Weight	19.6 kDa
Source	Recombinant DNA expressed in human cells
Purity	Greater than 95% as determined by ion exchange, hydrophobic interaction and/or size exclusion chromatographyEndotoxin level < 1 EU/μg
Bioactivity	Measured in cytopathic effect inhibition assay on human lung carcinoma cell line A549 with encephalomyocarditis virus (EMCV), EC₅₀ ~1 U/ml
Storage	For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Synonyms	Hu-IL-28B; Hu-IFN-λ3
Accession Number	NP_742151.2

Tech Info / Data

Titration of Human Interleukin-28B/Interferon Lambda 3

Background:

Interferon lambda 1 (IL-29), interferon lambda 2 (IL-28A) and interferon lambda 3 (IL-28B) are recently identified members of the IL-10 family, which have antiviral activity similar to type I interferons. They mediate this activity through a heterodimeric receptor complex that is composed of the IL-10 receptor β (IL-10 Rβ) and a novel IL-28 receptor α (IL-28 Rα, also known as IFN-λ R1). Engagement of this receptor leads to Jak-STAT signaling and gene expression. Many of the effects of interferon lambda on cells overlap that of Type I interferon. Unlike the type I interferon receptor which is on virtually all cells, the type III receptor is limited in its expression1. Interferon lambda 1 encodes a 181 AA mature protein which is 67% and 69% identical with interferon lambda 2 and interferon lambda 3, respectively.