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商品详细PBL/小鼠干扰素αA/1 x 105单位/12100-1
PBL/小鼠干扰素αA/1 x 105单位/12100-1
PBL/小鼠干扰素αA/1 x 105单位/12100-1
商品编号: 12100-1
品牌: PBL
市场价: ¥7900.00
美元价: 3950.00
产地: 美国(厂家直采)
公司:
产品分类: 超净台
公司分类: super_clean_bench
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Mouse IFN Alpha A

Product Features:
  • Mouse IFN Alpha A recombinant protein with activity > 1 x 105 U/ml
  • Distinct IFN-alpha subtype used extensively for research
  • Greater than 95% purity

Ordering

Catalog No. Pack Size Price Quantity
12100-1

1 x 105 units

$395.00
  • Specifications
  • Tech Info / Data
  • Citations
  • Documentation

Specifications

FormulationSupplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Molecular Weight19.3 kDa
SourcecDNA obtained from mouse leukocyte interferon expressed in E.coli
Purity> 95% by SDS-PAGE stained by Coomassie BlueEndotoxin level < 1 EU/μg
BioactivityMeasured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.EC50 for IFN Alpha A in this assay is ~ 5 U/ml.
StorageFor retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
SynonymsMouse Interferon Alpha, IFNA3, Interferon Alpha 3, IFNA, IFN-alpha A
Accession NumberNP_996753

Tech Info / Data

Titration of Mouse Interferon Alpha A in the L929 Cell Line
Background:

For more information on Mouse IFN Alpha subtype nomenclature click here.


Most mammalian species have multiple IFN-alpha subtypes. Although the reasons for these multiple subtypes are not fully known, there are clear cell type and temporal differences in their expression. A recent study established a nomenclature for the mouse IFN-alpha subtypes (van Pesch, et al. 2004) and determined relative activities of the subtypes with protein quantification by phosphorimaging of metabolically-labeled protein. In this study, Mouse IFN-alpha A was deemed to have average antiviral activity when compared with the potency of the other subtypes.

Mouse IFN-alpha A was initially cloned by Daugherty, et al. (1984) J. Interferon Res. 4(4) 635]. Being the first commercially available Mouse IFN-alpha protein, it has been used extensively in published research reports. Expression of this subtype has been observed in neurons infected with Theiler’s virus [Delhaye, et al. (2006) PNAS 103(20)7835]. Additionally, the protein has been shown to sensitize lymphocytes to undergo apoptosis [Cuerro, et al. (2004) J. Exp. Med. 200(4)535] and enhance the proliferation and differentiation of CD8 T-cells [Curtsinger, et al. (2005) J. Immunol. 174(8) 4465]. Furthermore, it was used to determine that B10.Q/J mice are susceptible to infection due to a Tyk2 mutation [Shaw, et al.(2003) PNAS 100(20)11594]. Hardy, et al. [Genomics (2004) 84:331] recently suggested a name of IFN Alpha 3 based on sequence comparison of the Mouse IFN-alpha subtype family of proteins.

Citations

Documentation

Antiviral Activities of PBL"s Mouse IFN Alpha A (12100) and Mouse IFN Alpha 4 (12115) on Mouse L929 Cells Infected with EMCV

 

Image depicting the Antiviral Activities of Mouse Interferon Alpha A and Interferon Alpha 4 as determined in an L929/EMCV CPE assay

The activity of Mouse IFN Alpha A and Mouse IFN Alpha 4 was compared in an L929/EMCV CPE assay. The EC50 for Mouse Alpha A in this experiment was 54 pg/ml.

Results are representative and may vary depending upon experimental conditions.

品牌介绍
来自PBL的iLite细胞基于常规的基因报告基因检测方法,并通过使用靶标特异性报告基因构建体进行工程改造。它们为定量测定生物活性干扰素水平提供了一种简单且经济高效的方法。