Mouse IFN Alpha 1
- Specifications
- Tech Info / Data
- Citations
- Documentation
Specifications
| Formulation | Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA) |
| Molecular Weight | 19.1 kDa |
| Source | Gene obtained from mouse DNA expressed in E.coli |
| Purity | > 95% by SDS-PAGE stained by Coomassie BlueEndotoxin level < 1 EU/μg |
| Bioactivity | Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.The EC50 for IFN in this assay is ~ 5 U/ml. |
| Storage | For retention of full activity store at -70oC or below and avoid repeated freeze/thaw cycles |
| Synonyms | Mu IFN-α1, Mouse IFN Alpha Subtype, Mouse IFN Protein Subtype, Interferon Alpha, IFN Alpha, IFN-a, Type I IFN Alpha |
| Accession Number | AY225950 |
Tech Info / Data
Mouse IFN Alpha 1 was originally cloned from a genomic library by Zwarthoff et al. [(1985) Nuc. Acids Res. 13(3)791]. It is produced by macrophages, neutrophils, plasmacytoid dendritic cells (pDCs) and other somatic cells in response to many viral and some pathogen infections.
Expression of the Mouse IFN Alpha 1 subtype has been seen in embryonic fibroblasts in some studies, but not in others. Expression has also been observed in brain and in L929 cells challenged with a variety of viruses [Yeow et al. (1997) Int. J. Biochem. Cell Biol. 29(3):519, Barchet et al., (2002) J. Exp. Med. 195(4):507, Delhaye et al., (2006) PNAS. 103(20):7835, Fung et al., (2004) J. Imm. Meth. 284:177]. It has been suggested that expression of this IFN may be regulated by Oct-1 and that it is IRF-7 dependent [Mesplede et al., (2005) Mol. Cell. 25(19):8717, Sato et al., (2000) Immunity 13:539].
This subtype has been extensively studied either in transgenic mice or by delivery as an expression construct [Hekman et al., (1988) J. Biol. Chem. 263(24):12151, Carr et al., (1988) J. Immunol. 169(9):5859]. When delivered as an expression construct, Mouse IFN Alpha 1 reduced CMV intramuscular infection significantly better than Mouse IFN Alpha 4 or 9 [Yeow et al., (1988) J. Immunol. 160(6):2932].
Summary: Mouse IFN Alpha 1 is an interferon with broad tissue expression which may be highly effective against certain viruses such as CMV and HSV.
For more information on Mouse IFN Alpha subtype nomenclature click here.
Citations
Documentation
12105-1 Certificate of Analysis (CoA) SDSComparison of Mouse IFN Alpha 1 and Mouse IFN Alpha A Antiviral Activity
The activity of Mouse IFN Alpha 1 and Mouse IFN Alpha A was compared in an L929/EMCV CPE assay. The EC50 for Mouse IFN Alpha 1 in this experiment was 62 pg/ml.
Results are representative and may vary depending upon experimental conditions.
