Human IFN Beta TCM ELISA, High Sensitivity
- Specifications
- Tech Info / Data
- Documentation
Specifications
Compatibility: Tissue Culture Media (TCM) onlyELISA Assay Range: 2.34 - 150 pg/mlSpeed: Assay time 3 hoursSpecificity: Human IFN Beta 1a & 1b, No cross-reactivity with human IFN-alpha, IFN-gamma, IFN-lambda, IL-6; mouse IFN-alpha, IFN-beta; or rat IFN-betaSynonyms: Human Beta Interferon, Human Fibroblast IFN, Human Type I Interferon Beta, Human IFNb
Inter-Assay CV: < 8%Intra-Assay CV: < 10%
Storage: 2-8 ºCExpiration Date: 12 months from date of manufactureShipping Condition: Wet Ice
Materials Provided:
- Pre-coated microtiter plate
- Plate Sealers
- Wash Solution Concentrate
- Human IFN Beta 1a Standard, 100,000 pg/ml
- Standard Diluent
- Sample Buffer
- Antibody Concentrate
- HRP Conjugate Concentrate
- Assay Diluent
- TMB Substrate
- Stop Solution
Tech Info / Data
Detailed Related Product Information:
- Quantitative Immunoassays: Single Analyte Screening Services
The VeriKine-HS Human IFN-Beta TCM ELISA Kit is designed for accurate quantification of low level human interferon beta in buffers or tissue culture media (TCM) by sandwich ELISA. The lowest concentration of Hu IFN-Beta is 2.34 pg/ml. This kit is not intended for use with human serum or plasma samples due to a significantly elevated risk of false positive results. For complex biological matrices such as plasma, healthy, and disease sera, we recommend our High Sensitivity Human IFN Beta Serum ELISA Kit (Cat. No. 41415-1).
Interferon Beta (IFN-Beta, IFNb) is synthesized and secreted by fibroblasts and many other cell types in response to varied stimuli in Toll-like receptor (TLR) dependent and independent mechanisms. Pathogen exposure can result in the activation of transcription factors interferon regulatory factor 3 (IRF3) and NF-kB resulting in IFN-Beta production in a stimuli and cell-dependent manner. Conversely, IFN-Beta aberrant expression may be associated with inflammation and autoimmunity.
Documentation
41435 Certificate of Analysis & Protocol (One-Page) 41435 Technical Data Sheet Safety Data Sheet (SDS) 41435 FlyerParallelism of Endogenous Samples using PBL"s High Sensitivity Human IFN-Beta TCM ELISA (41435)
Five endogenous samples were tested in duplicate for assay parallelism. Samples were serially diluted in TCM to the 32-fold dilution that lies within the kit"s assay range. Percent recoveries are 100 ± 20% of neat value except for the 32nd dilution of Sample 5 (S5) which falls marginally out of the assay"s LLOQ (2.34 pg/ml).
