>
产品中心 >
Small_molecule >
PBL/Human IFN Alpha All Subtype ELISA Kit, High Sensitivity (Serum, EDTA Plasma, TCM)/1 x 96-well plate/41115-1
Human IFN Alpha All Subtype ELISA Kit, High Sensitivity (Serum, EDTA Plasma, TCM)
- Specifications
- Tech Info / Data
- Citations
- Documentation
Specifications
Compatibility: Serum, EDTA Plasma, Tissue Culture MediaELISA Assay Range: 1.95 - 125 pg/mlSpeed: Typical Incubation time, 22 hours, 30 minutesSpecificity: Human Interferon AlphaCross-reacts with Cynomolgus/Rhesus IFN-α. No cross-reactivity against human IFN-β, IFN-γ, or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; or bovine IFN-τ.Synonyms: IFN alpha, IFN-a, Multisubtype Interferon Alpha, Multisubtype Leukocyte Interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha
Inter-Assay CV: ≤ 10%Intra-Assay CV: ≤ 8%Spike Recovery: ≥ 80% in Serum
Storage: 2-8°CExpiration Date: One year from date of manufactureShipping Condition: Wet Ice
Materials Provided:
- Pre-coated microtiter plate(s)
- Plate Sealers
- Wash Solution Concentrate
- Human Interferon Alpha Standard, 10,000 pg/ml
- Sample Buffer
- Standard Diluent
- Antibody Concentrate
- HRP Conjugate Concentrate
- Antibody Diluent
- HRP Diluent
- TMB Substrate
- Stop Solution
Tech Info / Data
Tips, Tools and Troubleshooting:
- Human IFN-α ELISA Subtype Specificities Comparison Chart
Detailed Related Product Information:
- Quantitative Immunoassays: Single Analyte Screening Services
This high sensitivity Human IFN Alpha ELISA kit is designed to provide accurate low pg/ml measurement of ALL human interferon alpha subtypes in autoimmune sera, normal serum, EDTA plasma, and tissue culture media (TCM). This unique feature provides an improved view of global IFN-alpha production in sample analysis.
Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. They are synthesized and secreted by most cell types in response to pathogens. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory and many other activities.
In humans, IFN-α consists of a group of proteins that are greater than 85% homologous by amino acid sequence. Numerous individual human IFN-α subtypes have been identified; many display different properties. It remains unclear why there are multiple IFN-α subtypes. A variety of studies suggested they possess overlapping but also unique sets of biological activities.
Citations
Documentation
41115 Protocol 41115-1 Certificate of Analysis (CoA) Product Flyer Safety Data Sheet (SDS)Representative Lower Limits of Quantitation and Lower Limits of Detection (pg/ml) in Normal Human Serum for each Human Interferon Alpha Subtype using PBL"s High Sensitivity Human IFN Alpha ELISA (41115)
Lower Limit of Quantitation (LLOQ) and Lower Limit of Detection (LLOD) for 41115
