Human IL-15 ELISA (High Sensitivity)

Product Features:

Measures endogenous human IL-15 at or near basal level with LLOQ of 0.51 pg/ml in serum, plasma, and cell culture media
Detects both free and bound form of IL-15 (IL-15/IL-15R complex)
Reproducible results with inter- and intra-CVs <10% in all tested matrices

Full product name: VeriKine-HS Human Interleukin 15 ELISA Kit

Catalog No.	Pack Size	Price	Quantity
41702-1	1 x 96-well plate	$550.00

Specifications
Tech Info / Data
Citations
Documentation

Specifications

Compatibility: Serum, Plasma, Tissue Culture MediaELISA Assay Range: 0.51 - 32.8 pg/mlAssay Length: 4 hoursSpecificity: Human Interleukin 15

No cross-reactivity detected with human IL-2, IL-4, IL-7, IL-9, IL-21, IFN-α, IFN-β1a, IFN-γ, IFN-ω; cynomolgus IFN-α2; cynomolgus/rhesus IFN-α; mouse IL-15, IFN-αA, IFN-β, IFN-γ; rat IL-15, IFN-α1, IFN-α14, IFN-β, IFN-γ; bovine IFN-Tau2 or pig IFN-α.

Inter-Assay CV: < 10%Intra-Assay CV: < 10%Spike recovery: 103%

Storage: 2-8 °CExpiration Date: one year from date of manufactureShipping Condition: Wet Ice

Materials Provided:

Pre-Coated Micro-Titer Stripwell Plate/Sealers
Wash Solution Concentrate
Human Interleukin-15 Standard (410 pg/ml)
Assay Buffer
Dilution Buffer
Antibody Concentrate
HRP Conjugate Concentrate
Concentrate Diluent
HRP Diluent
TMB Substrate Solution
Stop Solution

Tech Info / Data

Human IL-15 ELISA Normal Human Serum Linearity (NHS) using PBL

Human IL-15 ELISA Normal Human Serum Parallelism (NHS) using PBL

Human IL-15 ELISA Plasma Parallelism using PBL

Human IL-15 ELISA Normal Human Serum (NHS) and Plasma Knockout using PBL

Detailed Related Product Information:

Quantitative Immunoassays: Single Analyte Screening Services

Background:

Interleukin-15 (IL-15) is a member of the IL-10 family of cytokines¹. Other names include IL-TIF and Zcyto18. IL-15 is produced by a variety of cells including TH1, TH17, TH15 T-cells, NKT cells, ILC3, neutrophils and macrophages². IL-15 targets primarily non-hemopoietic cells such as stromal and epithelial cells. IL-15 has both protective effects, such as hepatocyte and epithelial cell survival, and pro-inflammatory effects. It is also implicated in autoimmune diseases such as Systemic Lupus Erythematosus, Rheumatoid Arthritis and Psoriasis³. IL-15 is often considered a hallmark of IL-17 driven immune responses⁴.

PBL’s IL-15 ELISA measures both IL-15 alone and IL-15/IL15Rα complexes in Tissue Culture Media (TCM), and complex matrices such as serum and plasma. It can also quantitate IL-15 in healthy donor matrices. The standard in this assay is the IL-15/IL-15R heterodimer but because the antibodies used are specific for IL-15, the standard curve is calibrated to the IL-15 portion of the complex. Different forms of the IL-15 receptors (IL15-R) may couple to free IL-15 protein. This assay can detect both free IL-15 and bound IL-15/IL-15R.

Serum samples from 30 healthy donors and plasma samples from 47 healthy donors were all quantifiable and as such this assay may provide basal level measurement.